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KMID : 0381919970270020197
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Korean Journal of Microscopy
1997 Volume.27 No. 2 p.197 ~ p.215
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Effect of Butylated Hydroxytoluene on Experimental Hepatocarcinogenesis Induced by 3’-Methyl-4-Dimethylaminoazobenzene I. Ultrastructural study
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Choi Jeung-Mok
Kang Dae-Young
Suh Kwang-Sun
Lee Choong-Sik
Song Kyu-Sang
Kim Jin-Man
Park Won-Hark
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Abstract
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A morphologic study on the effect of butylated hydroxytoluene (BHT) on experimental hepatocarcinogenesis induced by 3’’-methyl-4-dimethyl aminoazobenzene (3’’-MeDAB) was investigated. A total of 110 Sprague-Dowley male rats weighting about 200 g each were used for the experiment, and divided into 4 groups; the 3’’-MeDAB, BHT, 3’’-MeDAB/BHT treated group, and the control group. Four to eight rats of each group were sacrified on the 4th, 8th, 14th and 16th experimental weeks, with continuous pelletized feeding containing 0.09% 3’’-MeDAB and 0.5% BHT. The liver was examined by transmission and scanning electron microscopy. The results were as follows; Electron microscopically, the fine structure of the hepatocytes remained consistently abnormal up to 16 weeks after the 3’’-MeDAB treatment. There was no significant difference in the groups observed earlier than in the ones observed later. Many subcellular changes were observed : nuclear change, decreased glycogen, mitochondrial abnormalities, disaggregated rough endoplasmic reticulum, marked proliferation of smooth endoplasmic reticulum, dilatation and distortion of bile canaliculi, increased lysosomes, apoptotic bodies, migration of bile ductule cell. In the BHT treated group, the ultrastructural changes of hepatocytes were not significant, except for the lipid droplets and proliferated smooth endoplasmic reticulum among hepatocytes depending on the experimental duration. The various subcellular changes of 3’’-MeDAB/BHT treated groups were simillar to those of the 3’’-MeDAB treated group, but the degree of changes in the 3’’-MeDAB/ BHT treated group decreased compared with those of the 3’’-MeDAB treated group. These results suggest that dietary butylated hydroxytoluene has a protective/inhibitory effect on the hepatocarcinogenesis induced by 3’’-methyl-4-dimethyl -aminoazobenzene.
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KEYWORD
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3’-MeDAB, BHT Hepatocarcinogenesis, Ultrastructures
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